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About

  • Introduction
  • What is Nextstrain?
  • Parts of a whole
  • Augur → Auspice
  • Interpreting analyses
    • Phylogenetic trees
    • Phylogeographic map
    • Interaction
  • Pathogens
  • Nextstrain Groups

Tutorials

  • Installing
  • Running a phylogenetic workflow
  • Creating a phylogenetic workflow
  • Exploring SARS-CoV-2 evolution
  • Creating a phylogenetic workflow with VCF input
  • Writing a narrative
  • Analyzing genomes with Nextclade
  • Using a pathogen repository
    • Running an ingest workflow
  • Creating a pathogen repository
    • Creating an ingest workflow

How-to Guides

  • Bioinformatics
    • Filtering and Subsampling
    • Augur and snakemake
    • Missing sequence data (gaps, indels, ambiguity)
    • Finding a translate and align reference file
    • Understanding root and reference sequences
    • Importing BEAST MCC trees into augur
    • Manually Labeling Clades on a Nextstrain Tree
    • Adding Custom Trait Colors
    • Adding Custom Lat-Long Data
    • Using VCF Input
    • Using Fasta Input
    • Inferring Sequence Traits (like Drug Resistance)
    • Examples of Augur in the wild
  • Sharing analyses
    • Nextstrain Groups
      • Customize your page
    • Nextstrain Community
    • Public URLs
    • SARS-CoV-2 datasets
    • Downloading data
  • Communicating scientific insights
    • Communicating Results Using Narratives
    • Converting a narrative to PDF
  • Using Pathoplexus in Nextstrain
  • Viewing previous analyses
  • Managing an installation
  • Contributing

Components

  • Augur
  • Auspice
  • Nextstrain CLI
  • Nextclade CLI
  • nextstrain.org

Reference

  • Glossary
  • Data formats
  • Data files
  • FAQ
  • CA certificate trust stores
  • Snakemake style guide
  • Documentation style guide
  • Governance
Nextstrain
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Running Bioinformatic Analyses

How-to guides for performing bioinformatic anaylses with Nextstrain.

Table of contents

  • Filtering and Subsampling
  • Augur and snakemake
  • Missing sequence data (gaps, indels, ambiguity)
  • Finding a translate and align reference file
  • Understanding root and reference sequences
  • Importing BEAST MCC trees into augur
  • Manually Labeling Clades on a Nextstrain Tree
  • Adding Custom Trait Colors
  • Adding Custom Lat-Long Data
  • Using VCF Input
  • Using Fasta Input
  • Inferring Sequence Traits (like Drug Resistance)
  • Examples of Augur in the wild
Previous Next

Hadfield et al., Nextstrain: real-time tracking of pathogen evolution , Bioinformatics (2018)

The core Nextstrain team is

Trevor Bedford , Richard Neher , Ivan Aksamentov , Kim Andrews , Jennifer Chang , James Hadfield , Emma Hodcroft , John Huddleston , Jover Lee , Victor Lin , Cornelius Roemer

Please see the team page for more details.


All source code for Nextstrain is freely available under the terms of an open-source license, typically AGPL-3.0 or MIT. Refer to specific projects for details. Screenshots may be used under a CC-BY-4.0 license and attribution to nextstrain.org must be provided.
This work is made possible by the open sharing of genetic data by research groups from all over the world. We gratefully acknowledge their contributions. Special thanks to Kristian Andersen, Josh Batson, David Blazes, Jesse Bloom, Peter Bogner, Anderson Brito, Matt Cotten, Ana Crisan, Tulio de Oliveira, Gytis Dudas, Vivien Dugan, Karl Erlandson, Nuno Faria, Jennifer Gardy, Nate Grubaugh, Becky Kondor, Dylan George, Ian Goodfellow, Betz Halloran, Christian Happi, Jeff Joy, Paul Kellam, Philippe Lemey, Nick Loman, Duncan MacCannell, Erick Matsen, Sebastian Maurer-Stroh, Placide Mbala, Danny Park, Oliver Pybus, Andrew Rambaut, Colin Russell, Pardis Sabeti, Katherine Siddle, Kristof Theys, Dave Wentworth, Shirlee Wohl and Cecile Viboud for comments, suggestions and data sharing.

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© Copyright 2026, Trevor Bedford and Richard Neher

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